ssr_primer
Primer Details for NODE_164149_length_1266_cov_34.574183 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCCACACATTGTTGACGG | ATGTTCGACGTGGACAGCTT | Start: 963 |
| Tm | 60.04 | 59.966 | End: 1081 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CAGCCACACATTGTTGACGG | CTGGACATGTTCGACGTGGA | Start: 963 |
| Tm | 60.04 | 60.039 | End: 1087 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCTCGCCCTTTCTCTCTCTC | ATGTTCGACGTGGACAGCTT | Start: 887 |
| Tm | 59.898 | 59.966 | End: 1081 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
|---|
| Sequence | GCACACAGCCACACATTGTT | ATGTTCGACGTGGACAGCTT | Start: 958 |
| Tm | 59.897 | 59.966 | End: 1081 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TTCGCACCTCTTTCTCACCC | GAGAGAGAGAAAGGGCGAGC | Start: 670 |
| Tm | 59.965 | 59.898 | End: 906 |
| Size | 20 | 20 | 237 |
(CT)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGCCACACATTGTTGACGG | ATGTTCGACGTGGACAGCTT | Start: 963 |
| Tm | 60.04 | 59.966 | End: 1081 |
| Size | 20 | 20 | 119 |
| Primer 2 details |
|---|
| Sequence | CAGCCACACATTGTTGACGG | CTGGACATGTTCGACGTGGA | Start: 963 |
| Tm | 60.04 | 60.039 | End: 1087 |
| Size | 20 | 20 | 125 |
| Primer 3 details |
|---|
| Sequence | GCTCGCCCTTTCTCTCTCTC | ATGTTCGACGTGGACAGCTT | Start: 887 |
| Tm | 59.898 | 59.966 | End: 1081 |
| Size | 20 | 20 | 195 |
| Primer 4 details |
|---|
| Sequence | GCACACAGCCACACATTGTT | ATGTTCGACGTGGACAGCTT | Start: 958 |
| Tm | 59.897 | 59.966 | End: 1081 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TTCGCACCTCTTTCTCACCC | GAGAGAGAGAAAGGGCGAGC | Start: 670 |
| Tm | 59.965 | 59.898 | End: 906 |
| Size | 20 | 20 | 237 |
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