ssr_primer
Primer Details for NODE_163533_length_2311_cov_4.923592 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTTCCCCAAGTCCATCAA | TCACACACTTGTACTCGCCC | Start: 287 |
| Tm | 59.96 | 59.967 | End: 446 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCCTTCCCCAAGTCCATCAA | ACACACTTGTACTCGCCCAG | Start: 287 |
| Tm | 59.96 | 59.966 | End: 444 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | GCCTTCCCCAAGTCCATCAA | ACGTGGTCGACTTTGATGCT | Start: 287 |
| Tm | 59.96 | 59.966 | End: 495 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | TCACCATCAAAAGCGTTGCG | ACGTGGTCGACTTTGATGCT | Start: 396 |
| Tm | 60.041 | 59.966 | End: 495 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | CTACTGGGTCAGAGGGGACA | TCACACACTTGTACTCGCCC | Start: 307 |
| Tm | 59.959 | 59.967 | End: 446 |
| Size | 20 | 20 | 140 |
(A)10taatgcaaggtaatgcaaggatgaaattttatgaaatttt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCTTCCCCAAGTCCATCAA | TCACACACTTGTACTCGCCC | Start: 287 |
| Tm | 59.96 | 59.967 | End: 446 |
| Size | 20 | 20 | 160 |
| Primer 2 details |
|---|
| Sequence | GCCTTCCCCAAGTCCATCAA | ACACACTTGTACTCGCCCAG | Start: 287 |
| Tm | 59.96 | 59.966 | End: 444 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | GCCTTCCCCAAGTCCATCAA | ACGTGGTCGACTTTGATGCT | Start: 287 |
| Tm | 59.96 | 59.966 | End: 495 |
| Size | 20 | 20 | 209 |
| Primer 4 details |
|---|
| Sequence | TCACCATCAAAAGCGTTGCG | ACGTGGTCGACTTTGATGCT | Start: 396 |
| Tm | 60.041 | 59.966 | End: 495 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | CTACTGGGTCAGAGGGGACA | TCACACACTTGTACTCGCCC | Start: 307 |
| Tm | 59.959 | 59.967 | End: 446 |
| Size | 20 | 20 | 140 |
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