ssr_primer
Primer Details for NODE_161933_length_1718_cov_63.829179 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAGGGGCCTTTGCTATCC | AACATTCACTTGGCGCCCTA | Start: 1127 |
| Tm | 60.033 | 59.962 | End: 1366 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | AGTATCGCCGGAGCTCAAAG | GTCTTGAACGGCCGAGAGAA | Start: 228 |
| Tm | 59.897 | 60.039 | End: 373 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | GGAACTCTTCTGGGGTGCAA | GGATAGCAAAGGCCCCTGTT | Start: 1039 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTGGAACTCTTCTGGGGTGC | GGATAGCAAAGGCCCCTGTT | Start: 1037 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GCCTTTGCTATCCTCCTGGG | AACATTCACTTGGCGCCCTA | Start: 1134 |
| Tm | 60.179 | 59.962 | End: 1366 |
| Size | 20 | 20 | 233 |
(AT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAGGGGCCTTTGCTATCC | AACATTCACTTGGCGCCCTA | Start: 1127 |
| Tm | 60.033 | 59.962 | End: 1366 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | AGTATCGCCGGAGCTCAAAG | GTCTTGAACGGCCGAGAGAA | Start: 228 |
| Tm | 59.897 | 60.039 | End: 373 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | GGAACTCTTCTGGGGTGCAA | GGATAGCAAAGGCCCCTGTT | Start: 1039 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTGGAACTCTTCTGGGGTGC | GGATAGCAAAGGCCCCTGTT | Start: 1037 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GCCTTTGCTATCCTCCTGGG | AACATTCACTTGGCGCCCTA | Start: 1134 |
| Tm | 60.179 | 59.962 | End: 1366 |
| Size | 20 | 20 | 233 |
(A)11ttataacacattataacacagaccaacagagaccaacag(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACAGGGGCCTTTGCTATCC | AACATTCACTTGGCGCCCTA | Start: 1127 |
| Tm | 60.033 | 59.962 | End: 1366 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
|---|
| Sequence | AGTATCGCCGGAGCTCAAAG | GTCTTGAACGGCCGAGAGAA | Start: 228 |
| Tm | 59.897 | 60.039 | End: 373 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
|---|
| Sequence | GGAACTCTTCTGGGGTGCAA | GGATAGCAAAGGCCCCTGTT | Start: 1039 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | TTGGAACTCTTCTGGGGTGC | GGATAGCAAAGGCCCCTGTT | Start: 1037 |
| Tm | 59.89 | 60.033 | End: 1146 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
|---|
| Sequence | GCCTTTGCTATCCTCCTGGG | AACATTCACTTGGCGCCCTA | Start: 1134 |
| Tm | 60.179 | 59.962 | End: 1366 |
| Size | 20 | 20 | 233 |
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