ssr_primer
Primer Details for NODE_161843_length_1005_cov_19.436695 |
|---|
| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTCAAAGCCCCCTTGA | TGACAGACGACCAAAAGCCT | Start: 394 |
| Tm | 60.179 | 59.532 | End: 556 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
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| Sequence | AGGCTTTTGGTCGTCTGTCA | TTCCAACAGGTGTTTCGGCA | Start: 537 |
| Tm | 59.532 | 60.394 | End: 710 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | GCATTCAAAGCCCCCTTGAG | TGACAGACGACCAAAAGCCT | Start: 395 |
| Tm | 59.466 | 59.532 | End: 556 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTGCATTCAAAGCCCCCTTG | TGACAGACGACCAAAAGCCT | Start: 393 |
| Tm | 59.314 | 59.532 | End: 556 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | GTTTGCATTCAAAGCCCCCT | TGACAGACGACCAAAAGCCT | Start: 391 |
| Tm | 59.313 | 59.532 | End: 556 |
| Size | 20 | 20 | 166 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTCAAAGCCCCCTTGA | TGACAGACGACCAAAAGCCT | Start: 394 |
| Tm | 60.179 | 59.532 | End: 556 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | AGGCTTTTGGTCGTCTGTCA | TTCCAACAGGTGTTTCGGCA | Start: 537 |
| Tm | 59.532 | 60.394 | End: 710 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | GCATTCAAAGCCCCCTTGAG | TGACAGACGACCAAAAGCCT | Start: 395 |
| Tm | 59.466 | 59.532 | End: 556 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTGCATTCAAAGCCCCCTTG | TGACAGACGACCAAAAGCCT | Start: 393 |
| Tm | 59.314 | 59.532 | End: 556 |
| Size | 20 | 20 | 164 |
| Primer 5 details |
|---|
| Sequence | GTTTGCATTCAAAGCCCCCT | TGACAGACGACCAAAAGCCT | Start: 391 |
| Tm | 59.313 | 59.532 | End: 556 |
| Size | 20 | 20 | 166 |
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