ssr_primer
Primer Details for NODE_161611_length_533_cov_1836.258696 |
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| (T)10gacgggatccgacgggatccataatgtttaataatgttta(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGGGAGATCAGGTCGCT | GCCAGGACCAACCAAAGACT | Start: 122 |
| Tm | 60.033 | 60.179 | End: 262 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
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| Sequence | AGAAGGGAGATCAGGTCGCT | TGATGCCAGGACCAACCAAA | Start: 122 |
| Tm | 60.033 | 59.813 | End: 266 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
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| Sequence | CAGAAGGGAGATCAGGTCGC | GCCAGGACCAACCAAAGACT | Start: 121 |
| Tm | 59.896 | 60.179 | End: 262 |
| Size | 20 | 20 | 142 |
| Primer 4 details |
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| Sequence | AGAAGGGAGATCAGGTCGCT | CTGATGCCAGGACCAACCAA | Start: 122 |
| Tm | 60.033 | 60.251 | End: 267 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
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| Sequence | GGGGAGGGGGAGGTTCTTAA | ATGCGCACATGCTGTTTGTT | Start: 412 |
| Tm | 60.253 | 59.968 | End: 528 |
| Size | 20 | 20 | 117 |
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