ssr_primer
Primer Details for NODE_161427_length_650_cov_5.431542 |
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| (T)10acacaaatatacacaaatatgtctttaattgtctttaa(T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCCGTCTTGACCCAACAC | CAGGACCACGTCGTGTACTC | Start: 332 |
| Tm | 59.894 | 60.11 | End: 553 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
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| Sequence | CAGATTGGCGTCCACCAGAT | CAGGACCACGTCGTGTACTC | Start: 362 |
| Tm | 60.108 | 60.11 | End: 553 |
| Size | 20 | 20 | 192 |
| Primer 3 details |
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| Sequence | CGTCCACGACACCGAAAATG | CAGGACCACGTCGTGTACTC | Start: 312 |
| Tm | 59.837 | 60.11 | End: 553 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
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| Sequence | AGTCCGTCTTGACCCAACAC | CCAGAACCCCTACGACTTCG | Start: 332 |
| Tm | 59.894 | 59.827 | End: 464 |
| Size | 20 | 20 | 133 |
| Primer 5 details |
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| Sequence | CAGATTGGCGTCCACCAGAT | CCAGAACCCCTACGACTTCG | Start: 362 |
| Tm | 60.108 | 59.827 | End: 464 |
| Size | 20 | 20 | 103 |
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