ssr_primer
Primer Details for NODE_160757_length_722_cov_81.725732 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTACGATGAGCTGGGAGAG | GTGTTGACCTCGAGGGAGTG | Start: 566 |
| Tm | 59.691 | 60.038 | End: 703 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | TGGAATTGGGGGCGTTAAGG | GTGTTGACCTCGAGGGAGTG | Start: 503 |
| Tm | 60.324 | 60.038 | End: 703 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | GCGATGGTGATGGTGGTGAT | GCTCTCCCAGCTCATCGTAC | Start: 345 |
| Tm | 60.464 | 59.969 | End: 586 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TTTTAACGGTGGCTCACGGT | ACGAGGAGCAAGCACTTCAT | Start: 5 |
| Tm | 60.179 | 59.677 | End: 173 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | GCTCACGGTGGCTCATGTAT | ACGAGGAGCAAGCACTTCAT | Start: 16 |
| Tm | 60.179 | 59.677 | End: 173 |
| Size | 20 | 20 | 158 |
(GCC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGTACGATGAGCTGGGAGAG | GTGTTGACCTCGAGGGAGTG | Start: 566 |
| Tm | 59.691 | 60.038 | End: 703 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | TGGAATTGGGGGCGTTAAGG | GTGTTGACCTCGAGGGAGTG | Start: 503 |
| Tm | 60.324 | 60.038 | End: 703 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
|---|
| Sequence | GCGATGGTGATGGTGGTGAT | GCTCTCCCAGCTCATCGTAC | Start: 345 |
| Tm | 60.464 | 59.969 | End: 586 |
| Size | 20 | 20 | 242 |
| Primer 4 details |
|---|
| Sequence | TTTTAACGGTGGCTCACGGT | ACGAGGAGCAAGCACTTCAT | Start: 5 |
| Tm | 60.179 | 59.677 | End: 173 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
|---|
| Sequence | GCTCACGGTGGCTCATGTAT | ACGAGGAGCAAGCACTTCAT | Start: 16 |
| Tm | 60.179 | 59.677 | End: 173 |
| Size | 20 | 20 | 158 |
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