ssr_primer
Primer Details for NODE_160739_length_2408_cov_12.313062 |
|---|
| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGCTCCTCTAGATGGCT | GGCGCTCAAGAACAAAAGCA | Start: 614 |
| Tm | 60.032 | 59.969 | End: 869 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
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| Sequence | GCCTTTTGACTCGCCGTTTT | GAGGGCGCTTGATACGATGA | Start: 2178 |
| Tm | 59.97 | 59.968 | End: 2400 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | GCCTTTTGACTCGCCGTTTT | AGAGGGCGCTTGATACGATG | Start: 2178 |
| Tm | 59.97 | 59.967 | End: 2401 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | TGCTTTTGTTCTTGAGCGCC | AACATGCGCAATCCTCCTCA | Start: 850 |
| Tm | 59.969 | 60.035 | End: 980 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
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| Sequence | TGCTTTTGTTCTTGAGCGCC | ATACACCTCCAACATGCGCA | Start: 850 |
| Tm | 59.969 | 60.036 | End: 990 |
| Size | 20 | 20 | 141 |
(T)11gtaattttttttctttttca(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTGCTCCTCTAGATGGCT | GGCGCTCAAGAACAAAAGCA | Start: 614 |
| Tm | 60.032 | 59.969 | End: 869 |
| Size | 20 | 20 | 256 |
| Primer 2 details |
|---|
| Sequence | GCCTTTTGACTCGCCGTTTT | GAGGGCGCTTGATACGATGA | Start: 2178 |
| Tm | 59.97 | 59.968 | End: 2400 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | GCCTTTTGACTCGCCGTTTT | AGAGGGCGCTTGATACGATG | Start: 2178 |
| Tm | 59.97 | 59.967 | End: 2401 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | TGCTTTTGTTCTTGAGCGCC | AACATGCGCAATCCTCCTCA | Start: 850 |
| Tm | 59.969 | 60.035 | End: 980 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | TGCTTTTGTTCTTGAGCGCC | ATACACCTCCAACATGCGCA | Start: 850 |
| Tm | 59.969 | 60.036 | End: 990 |
| Size | 20 | 20 | 141 |
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