Primer Details for NODE_160641_length_243_cov_25.070588 | |||
|---|---|---|---|
| (A)13ctatttaaaactatttaaaaactagtgaaaactagtg(A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGTCTTCTGTTTGTTTTTGTGCA | AGAGACAGTCACTGTAACAGGT | Start: 40 |
| Tm | 60.022 | 58.433 | End: 144 |
| Size | 24 | 22 | 105 |
| Primer 2 details | |||
| Sequence | GGTCTTCTGTTTGTTTTTGTGCA | AGAGACAGTCACTGTAACAGGT | Start: 41 |
| Tm | 58.758 | 58.433 | End: 144 |
| Size | 23 | 22 | 104 |
| Primer 3 details | |||
| Sequence | GGTCTTCTGTTTGTTTTTGTGCAG | AGAGACAGTCACTGTAACAGGT | Start: 41 |
| Tm | 59.673 | 58.433 | End: 144 |
| Size | 24 | 22 | 104 |
| Primer 4 details | |||
| Sequence | AGGTCTTCTGTTTGTTTTTGTGCA | AAGAGACAGTCACTGTAACAGGT | Start: 40 |
| Tm | 60.022 | 59.039 | End: 145 |
| Size | 24 | 23 | 106 |
| Primer 5 details | |||
| Sequence | TGTGCAGTGTACAAACCATGA | ACACAACATTCAAAGAGACAGTCAC | Start: 58 |
| Tm | 58.076 | 59.935 | End: 157 |
| Size | 21 | 25 | 100 |
