Primer Details for NODE_158853_length_538_cov_60.733333 | |||
|---|---|---|---|
| (T)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTTTTGTCAAACAACACACA | GGTCCTGTGCTGGCATCTTA | Start: 2 |
| Tm | 57.144 | 59.747 | End: 153 |
| Size | 22 | 20 | 152 |
| Primer 2 details | |||
| Sequence | ACAACACACAAATGTACAACACACT | GGTCCTGTGCTGGCATCTTA | Start: 14 |
| Tm | 60.048 | 59.747 | End: 153 |
| Size | 25 | 20 | 140 |
| Primer 3 details | |||
| Sequence | ACAACACACAAATGTACAACACAC | GGTCCTGTGCTGGCATCTTA | Start: 14 |
| Tm | 58.842 | 59.747 | End: 153 |
| Size | 24 | 20 | 140 |
| Primer 4 details | |||
| Sequence | TCCTTTTGTCAAACAACACACAA | GGTCCTGTGCTGGCATCTTA | Start: 2 |
| Tm | 57.798 | 59.747 | End: 153 |
| Size | 23 | 20 | 152 |
| Primer 5 details | |||
| Sequence | TTCCTTTTGTCAAACAACACACA | GGTCCTGTGCTGGCATCTTA | Start: 1 |
| Tm | 57.798 | 59.747 | End: 153 |
| Size | 23 | 20 | 153 |
