ssr_primer
Primer Details for NODE_158851_length_963_cov_11.844944 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGGTCACCACGGACTTAC | AGAGCTTATGTAGGCGTGCC | Start: 486 |
| Tm | 60.038 | 59.895 | End: 669 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | TCTCACCGTTTCCGAGCAAA | GCGGCACAGTTTCGGAAATT | Start: 48 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TTCTCACCGTTTCCGAGCAA | GCGGCACAGTTTCGGAAATT | Start: 47 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TTTCTCACCGTTTCCGAGCA | GCGGCACAGTTTCGGAAATT | Start: 46 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | GGCACGCCTACATAAGCTCT | ACACGTCGTTTGGTGGTTCT | Start: 650 |
| Tm | 59.895 | 60.108 | End: 802 |
| Size | 20 | 20 | 153 |
(T)10caagttaatggataaaagtggataaaagtgtttttttttg(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGGTCACCACGGACTTAC | AGAGCTTATGTAGGCGTGCC | Start: 486 |
| Tm | 60.038 | 59.895 | End: 669 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | TCTCACCGTTTCCGAGCAAA | GCGGCACAGTTTCGGAAATT | Start: 48 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | TTCTCACCGTTTCCGAGCAA | GCGGCACAGTTTCGGAAATT | Start: 47 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 114 |
| Primer 4 details |
|---|
| Sequence | TTTCTCACCGTTTCCGAGCA | GCGGCACAGTTTCGGAAATT | Start: 46 |
| Tm | 59.895 | 60.04 | End: 160 |
| Size | 20 | 20 | 115 |
| Primer 5 details |
|---|
| Sequence | GGCACGCCTACATAAGCTCT | ACACGTCGTTTGGTGGTTCT | Start: 650 |
| Tm | 59.895 | 60.108 | End: 802 |
| Size | 20 | 20 | 153 |
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