Primer Details for NODE_156981_length_210_cov_2025.430657 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACATCGCGGTAGCAATTTTT | TGCTACCTCGATGTTGAATTAAGA | Start: 74 |
| Tm | 57.431 | 58.027 | End: 208 |
| Size | 21 | 24 | 135 |
| Primer 2 details |
|---|
| Sequence | AACATCGCGGTAGCAATTTTT | TTGCTACCTCGATGTTGAATTAAGA | Start: 74 |
| Tm | 57.431 | 58.597 | End: 209 |
| Size | 21 | 25 | 136 |
| Primer 3 details |
|---|
| Sequence | AACATCGCGGTAGCAATTTTT | TGCTACCTCGATGTTGAATTAAGAT | Start: 74 |
| Tm | 57.431 | 58.417 | End: 208 |
| Size | 21 | 25 | 135 |
| Primer 4 details |
|---|
| Sequence | AACATCGCGGTAGCAATTTTT | TTGCTACCTCGATGTTGAATTAAG | Start: 74 |
| Tm | 57.431 | 57.338 | End: 209 |
| Size | 21 | 24 | 136 |
| Primer 5 details |
|---|
| Sequence | AACATCGCGGTAGCAATTTTT | TTGCTACCTCGATGTTGAATTAAGAT | Start: 74 |
| Tm | 57.431 | 58.952 | End: 209 |
| Size | 21 | 26 | 136 |
