ssr_primer
Primer Details for NODE_156125_length_643_cov_6.314035 |
|---|
| (GCCT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCCTTATCACCACAGTGC | CAAGATATCGCCGGCGTACT | Start: 98 |
| Tm | 60.035 | 60.039 | End: 300 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | AGGCCTTATCACCACAGTGC | TCAAGATATCGCCGGCGTAC | Start: 98 |
| Tm | 60.035 | 60.04 | End: 301 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
|---|
| Sequence | GCGTTTGACAGCCGAGATTC | CAAGATATCGCCGGCGTACT | Start: 176 |
| Tm | 59.905 | 60.039 | End: 300 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | GCGTTTGACAGCCGAGATTC | TCAAGATATCGCCGGCGTAC | Start: 176 |
| Tm | 59.905 | 60.04 | End: 301 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | GGAGAGCGTCTTAGAGGTGC | ACACACACACACACACCACT | Start: 492 |
| Tm | 59.898 | 59.964 | End: 627 |
| Size | 20 | 20 | 136 |
(TGT)5gcaaaaagtggccgtgtgttggcggcagtgtagtg(GT)7cgtgcagagtgtagtggagtgtagtg(GT)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCCTTATCACCACAGTGC | CAAGATATCGCCGGCGTACT | Start: 98 |
| Tm | 60.035 | 60.039 | End: 300 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | AGGCCTTATCACCACAGTGC | TCAAGATATCGCCGGCGTAC | Start: 98 |
| Tm | 60.035 | 60.04 | End: 301 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
|---|
| Sequence | GCGTTTGACAGCCGAGATTC | CAAGATATCGCCGGCGTACT | Start: 176 |
| Tm | 59.905 | 60.039 | End: 300 |
| Size | 20 | 20 | 125 |
| Primer 4 details |
|---|
| Sequence | GCGTTTGACAGCCGAGATTC | TCAAGATATCGCCGGCGTAC | Start: 176 |
| Tm | 59.905 | 60.04 | End: 301 |
| Size | 20 | 20 | 126 |
| Primer 5 details |
|---|
| Sequence | GGAGAGCGTCTTAGAGGTGC | ACACACACACACACACCACT | Start: 492 |
| Tm | 59.898 | 59.964 | End: 627 |
| Size | 20 | 20 | 136 |
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