ssr_primer
Primer Details for NODE_156068_length_1230_cov_18.547105 |
|---|
| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACAAGCACCTCGAGGAAT | GAGTACTGGCCCTTGACCAC | Start: 65 |
| Tm | 60.036 | 60.036 | End: 196 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CCCTCAAGAAGGTCCACCAC | GAGTACTGGCCCTTGACCAC | Start: 49 |
| Tm | 59.963 | 60.036 | End: 196 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | TGCTACATTGCCCGTCCTTT | Start: 177 |
| Tm | 60.036 | 59.962 | End: 379 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | CTGGAACCGCTACGACAACT | Start: 177 |
| Tm | 60.036 | 60.039 | End: 350 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | TCTGGAACCGCTACGACAAC | Start: 177 |
| Tm | 60.036 | 60.039 | End: 351 |
| Size | 20 | 20 | 175 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACAAGCACCTCGAGGAAT | GAGTACTGGCCCTTGACCAC | Start: 65 |
| Tm | 60.036 | 60.036 | End: 196 |
| Size | 20 | 20 | 132 |
| Primer 2 details |
|---|
| Sequence | CCCTCAAGAAGGTCCACCAC | GAGTACTGGCCCTTGACCAC | Start: 49 |
| Tm | 59.963 | 60.036 | End: 196 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | TGCTACATTGCCCGTCCTTT | Start: 177 |
| Tm | 60.036 | 59.962 | End: 379 |
| Size | 20 | 20 | 203 |
| Primer 4 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | CTGGAACCGCTACGACAACT | Start: 177 |
| Tm | 60.036 | 60.039 | End: 350 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | GTGGTCAAGGGCCAGTACTC | TCTGGAACCGCTACGACAAC | Start: 177 |
| Tm | 60.036 | 60.039 | End: 351 |
| Size | 20 | 20 | 175 |
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