ssr_primer
Primer Details for NODE_155132_length_913_cov_5.683333 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGGGCGTTTCGGAAAAAT | AACGGCGAAGGTGAAGTCAT | Start: 351 |
| Tm | 60.04 | 59.965 | End: 592 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | ATGACTTCACCTTCGCCGTT | TGGATCCTCCACCACCTCAT | Start: 573 |
| Tm | 59.965 | 59.955 | End: 809 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | GAAATTGTCCGTCGTCGCTG | AACGGCGAAGGTGAAGTCAT | Start: 321 |
| Tm | 59.907 | 59.965 | End: 592 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | ATGACTTCACCTTCGCCGTT | TCAGAAGTCGCGGTGGAAAA | Start: 573 |
| Tm | 59.965 | 59.895 | End: 718 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACCGCGACTTCTGA | TGGATCCTCCACCACCTCAT | Start: 699 |
| Tm | 59.895 | 59.955 | End: 809 |
| Size | 20 | 20 | 111 |
(A)10gaaaacttaaatagtttcttatgaggtggtggaggatccacaaaacttaagatttaaaaccaaaaatgg(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACGGGCGTTTCGGAAAAAT | AACGGCGAAGGTGAAGTCAT | Start: 351 |
| Tm | 60.04 | 59.965 | End: 592 |
| Size | 20 | 20 | 242 |
| Primer 2 details |
|---|
| Sequence | ATGACTTCACCTTCGCCGTT | TGGATCCTCCACCACCTCAT | Start: 573 |
| Tm | 59.965 | 59.955 | End: 809 |
| Size | 20 | 20 | 237 |
| Primer 3 details |
|---|
| Sequence | GAAATTGTCCGTCGTCGCTG | AACGGCGAAGGTGAAGTCAT | Start: 321 |
| Tm | 59.907 | 59.965 | End: 592 |
| Size | 20 | 20 | 272 |
| Primer 4 details |
|---|
| Sequence | ATGACTTCACCTTCGCCGTT | TCAGAAGTCGCGGTGGAAAA | Start: 573 |
| Tm | 59.965 | 59.895 | End: 718 |
| Size | 20 | 20 | 146 |
| Primer 5 details |
|---|
| Sequence | TTTTCCACCGCGACTTCTGA | TGGATCCTCCACCACCTCAT | Start: 699 |
| Tm | 59.895 | 59.955 | End: 809 |
| Size | 20 | 20 | 111 |
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