Primer Details for NODE_154980_length_236_cov_1.079755 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTTTAGTCTGCCCAAACTGTG | AGTTCTTCATCCCATTCTTTGAATCA | Start: 131 |
| Tm | 59.117 | 59.05 | End: 235 |
| Size | 23 | 26 | 105 |
| Primer 2 details |
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| Sequence | GTCTTTTAGTCTGCCCAAACTGT | AGTTCTTCATCCCATTCTTTGAATCA | Start: 130 |
| Tm | 59.117 | 59.05 | End: 235 |
| Size | 23 | 26 | 106 |
| Primer 3 details |
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| Sequence | TCTTTTAGTCTGCCCAAACTGTG | GTTCTTCATCCCATTCTTTGAATCA | Start: 131 |
| Tm | 59.117 | 57.838 | End: 234 |
| Size | 23 | 25 | 104 |
| Primer 4 details |
|---|
| Sequence | GTCTTTTAGTCTGCCCAAACTGT | GTTCTTCATCCCATTCTTTGAATCA | Start: 130 |
| Tm | 59.117 | 57.838 | End: 234 |
| Size | 23 | 25 | 105 |
| Primer 5 details |
|---|
| Sequence | AGTCTTTTAGTCTGCCCAAACTG | AGTTCTTCATCCCATTCTTTGAATCA | Start: 129 |
| Tm | 58.862 | 59.05 | End: 235 |
| Size | 23 | 26 | 107 |
(A)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTTTAGTCTGCCCAAACTGTG | AGTTCTTCATCCCATTCTTTGAATCA | Start: 131 |
| Tm | 59.117 | 59.05 | End: 235 |
| Size | 23 | 26 | 105 |
| Primer 2 details |
|---|
| Sequence | GTCTTTTAGTCTGCCCAAACTGT | AGTTCTTCATCCCATTCTTTGAATCA | Start: 130 |
| Tm | 59.117 | 59.05 | End: 235 |
| Size | 23 | 26 | 106 |
| Primer 3 details |
|---|
| Sequence | TCTTTTAGTCTGCCCAAACTGTG | GTTCTTCATCCCATTCTTTGAATCA | Start: 131 |
| Tm | 59.117 | 57.838 | End: 234 |
| Size | 23 | 25 | 104 |
| Primer 4 details |
|---|
| Sequence | GTCTTTTAGTCTGCCCAAACTGT | GTTCTTCATCCCATTCTTTGAATCA | Start: 130 |
| Tm | 59.117 | 57.838 | End: 234 |
| Size | 23 | 25 | 105 |
| Primer 5 details |
|---|
| Sequence | AGTCTTTTAGTCTGCCCAAACTG | AGTTCTTCATCCCATTCTTTGAATCA | Start: 129 |
| Tm | 58.862 | 59.05 | End: 235 |
| Size | 23 | 26 | 107 |
