ssr_primer
Primer Details for NODE_154190_length_2997_cov_28.614569 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAGTTGAAGCTGCTCAG | TTACACAGCCTAAACCGCGT | Start: 2151 |
| Tm | 59.964 | 59.966 | End: 2286 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | CAAGTACGGCCCCATGTTCT | GTGAACTCCTCCTTGCGGAA | Start: 393 |
| Tm | 60.035 | 59.965 | End: 494 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTGAGATGTGGTGGGTCTGC | TTACACAGCCTAAACCGCGT | Start: 2072 |
| Tm | 59.963 | 59.966 | End: 2286 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | GGGACTACATCGCACCAACA | TTACACAGCCTAAACCGCGT | Start: 2016 |
| Tm | 60.037 | 59.966 | End: 2286 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CCATACGGGGACTACATCGC | TTACACAGCCTAAACCGCGT | Start: 2009 |
| Tm | 60.039 | 59.966 | End: 2286 |
| Size | 20 | 20 | 278 |
(A)13tacagaaaaatacagaaaacctaattgaacctaattg(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAGTTGAAGCTGCTCAG | TTACACAGCCTAAACCGCGT | Start: 2151 |
| Tm | 59.964 | 59.966 | End: 2286 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | CAAGTACGGCCCCATGTTCT | GTGAACTCCTCCTTGCGGAA | Start: 393 |
| Tm | 60.035 | 59.965 | End: 494 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTGAGATGTGGTGGGTCTGC | TTACACAGCCTAAACCGCGT | Start: 2072 |
| Tm | 59.963 | 59.966 | End: 2286 |
| Size | 20 | 20 | 215 |
| Primer 4 details |
|---|
| Sequence | GGGACTACATCGCACCAACA | TTACACAGCCTAAACCGCGT | Start: 2016 |
| Tm | 60.037 | 59.966 | End: 2286 |
| Size | 20 | 20 | 271 |
| Primer 5 details |
|---|
| Sequence | CCATACGGGGACTACATCGC | TTACACAGCCTAAACCGCGT | Start: 2009 |
| Tm | 60.039 | 59.966 | End: 2286 |
| Size | 20 | 20 | 278 |
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