ssr_primer
Primer Details for NODE_151316_length_3057_cov_27.297252 |
|---|
| (TCA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACCTTCACCTACGCCAA | AGTAGAACGAGAGCGCGATG | Start: 524 |
| Tm | 59.966 | 59.972 | End: 665 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | ACACCTTCACCTACGCCAAG | AGTAGAACGAGAGCGCGATG | Start: 525 |
| Tm | 59.965 | 59.972 | End: 665 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CTACGTCGGCTTCGCTACAT | GGATGTAGGCGATGGGGAAG | Start: 1114 |
| Tm | 59.971 | 59.965 | End: 1241 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | CATCGCGCTCTCGTTCTACT | GACAGGGTGGTGTAGAAGGC | Start: 646 |
| Tm | 59.972 | 60.036 | End: 822 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | ACGTGCTCAAGCCCTTCTAC | AGTAGAACGAGAGCGCGATG | Start: 396 |
| Tm | 60.037 | 59.972 | End: 665 |
| Size | 20 | 20 | 270 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACACCTTCACCTACGCCAA | AGTAGAACGAGAGCGCGATG | Start: 524 |
| Tm | 59.966 | 59.972 | End: 665 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | ACACCTTCACCTACGCCAAG | AGTAGAACGAGAGCGCGATG | Start: 525 |
| Tm | 59.965 | 59.972 | End: 665 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | CTACGTCGGCTTCGCTACAT | GGATGTAGGCGATGGGGAAG | Start: 1114 |
| Tm | 59.971 | 59.965 | End: 1241 |
| Size | 20 | 20 | 128 |
| Primer 4 details |
|---|
| Sequence | CATCGCGCTCTCGTTCTACT | GACAGGGTGGTGTAGAAGGC | Start: 646 |
| Tm | 59.972 | 60.036 | End: 822 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | ACGTGCTCAAGCCCTTCTAC | AGTAGAACGAGAGCGCGATG | Start: 396 |
| Tm | 60.037 | 59.972 | End: 665 |
| Size | 20 | 20 | 270 |
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