ssr_primer
Primer Details for NODE_150401_length_408_cov_320.316418 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGGCATCAAATCAACAGG | ACACGGTGATCTGAGGAGGA | Start: 60 |
| Tm | 60.179 | 59.96 | End: 194 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | GCGGCATCAAATCAACAGGA | ACACGGTGATCTGAGGAGGA | Start: 61 |
| Tm | 59.189 | 59.96 | End: 194 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | AGGCGGCATCAAATCAACAG | ACACGGTGATCTGAGGAGGA | Start: 59 |
| Tm | 59.187 | 59.96 | End: 194 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | TCATCCTTAGGCGGCATCAA | ACACGGTGATCTGAGGAGGA | Start: 51 |
| Tm | 59.163 | 59.96 | End: 194 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTCATCCTTAGGCGGCATCA | ACACGGTGATCTGAGGAGGA | Start: 50 |
| Tm | 59.163 | 59.96 | End: 194 |
| Size | 20 | 20 | 145 |
(A)13taaaatgaaataaaatgtaaagttttctaaagttttc(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGGCATCAAATCAACAGG | ACACGGTGATCTGAGGAGGA | Start: 60 |
| Tm | 60.179 | 59.96 | End: 194 |
| Size | 20 | 20 | 135 |
| Primer 2 details |
|---|
| Sequence | GCGGCATCAAATCAACAGGA | ACACGGTGATCTGAGGAGGA | Start: 61 |
| Tm | 59.189 | 59.96 | End: 194 |
| Size | 20 | 20 | 134 |
| Primer 3 details |
|---|
| Sequence | AGGCGGCATCAAATCAACAG | ACACGGTGATCTGAGGAGGA | Start: 59 |
| Tm | 59.187 | 59.96 | End: 194 |
| Size | 20 | 20 | 136 |
| Primer 4 details |
|---|
| Sequence | TCATCCTTAGGCGGCATCAA | ACACGGTGATCTGAGGAGGA | Start: 51 |
| Tm | 59.163 | 59.96 | End: 194 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTCATCCTTAGGCGGCATCA | ACACGGTGATCTGAGGAGGA | Start: 50 |
| Tm | 59.163 | 59.96 | End: 194 |
| Size | 20 | 20 | 145 |
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