Primer Details for NODE_150240_length_440_cov_23.945504 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGTAGACCTTTGACATTGA | TGGCATATCCTACTCTTCCAGT | Start: 198 |
| Tm | 57.376 | 58.071 | End: 354 |
| Size | 22 | 22 | 157 |
| Primer 2 details | |||
| Sequence | AGCTGTAGACCTTTGACATTGAA | TGGCATATCCTACTCTTCCAGT | Start: 198 |
| Tm | 58.027 | 58.071 | End: 354 |
| Size | 23 | 22 | 157 |
| Primer 3 details | |||
| Sequence | AGCTGTAGACCTTTGACATTGA | TGGCATATCCTACTCTTCCAGTT | Start: 198 |
| Tm | 57.376 | 58.701 | End: 354 |
| Size | 22 | 23 | 157 |
| Primer 4 details | |||
| Sequence | GCATTGTGATTTCTAGCTGTAGACC | TGGCATATCCTACTCTTCCAGT | Start: 184 |
| Tm | 59.993 | 58.071 | End: 354 |
| Size | 25 | 22 | 171 |
| Primer 5 details | |||
| Sequence | TGTAGTAAGATCAGCAAAGCAGT | TGGCATATCCTACTCTTCCAGT | Start: 233 |
| Tm | 57.847 | 58.071 | End: 354 |
| Size | 23 | 22 | 122 |
