Primer Details for NODE_149828_length_268_cov_58.394872 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CATGTCAGAGGCCATGAGGG | ACTCGTCAAATTATGCCAAAAAGGA | Start: 4 |
| Tm | 60.179 | 59.757 | End: 140 |
| Size | 20 | 25 | 137 |
| Primer 2 details |
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| Sequence | TCAGAGGCCATGAGGGAGTT | ACTCGTCAAATTATGCCAAAAAGGA | Start: 8 |
| Tm | 60.253 | 59.757 | End: 140 |
| Size | 20 | 25 | 133 |
| Primer 3 details |
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| Sequence | CATGTCAGAGGCCATGAGGG | CGTCAAATTATGCCAAAAAGGAAGG | Start: 4 |
| Tm | 60.179 | 59.652 | End: 137 |
| Size | 20 | 25 | 134 |
| Primer 4 details |
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| Sequence | ATGTCAGAGGCCATGAGGGA | ACTCGTCAAATTATGCCAAAAAGGA | Start: 5 |
| Tm | 60.327 | 59.757 | End: 140 |
| Size | 20 | 25 | 136 |
| Primer 5 details |
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| Sequence | CATGTCAGAGGCCATGAGGG | AATTATGCCAAAAAGGAAGGACC | Start: 4 |
| Tm | 60.179 | 57.579 | End: 132 |
| Size | 20 | 23 | 129 |
