ssr_primer
Primer Details for NODE_148904_length_223_cov_1.653333 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGGCAGACAACTTCTCT | TGTCTGCCACTGAATTCGTTG | Start: 59 |
| Tm | 58.958 | 59.127 | End: 221 |
| Size | 21 | 21 | 163 |
| Primer 2 details |
|---|
| Sequence | TCAGTGGCAGACAACTTCTCT | GTCTGCCACTGAATTCGTTGA | Start: 59 |
| Tm | 58.958 | 58.857 | End: 220 |
| Size | 21 | 21 | 162 |
| Primer 3 details |
|---|
| Sequence | TCAGTGGCAGACAACCATTT | TGTCTGCCACTGAATTCGTTG | Start: 109 |
| Tm | 57.637 | 59.127 | End: 221 |
| Size | 20 | 21 | 113 |
| Primer 4 details |
|---|
| Sequence | CGAATTCAGTGGCAGACAACC | GCCACTGAATTCGTTGAAGAGA | Start: 104 |
| Tm | 59.802 | 58.933 | End: 216 |
| Size | 21 | 22 | 113 |
| Primer 5 details |
|---|
| Sequence | TCAGTGGCAGACAACTTCTCT | TGTCTGCCACTGAATTCGTT | Start: 59 |
| Tm | 58.958 | 57.745 | End: 221 |
| Size | 21 | 20 | 163 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGTGGCAGACAACTTCTCT | TGTCTGCCACTGAATTCGTTG | Start: 59 |
| Tm | 58.958 | 59.127 | End: 221 |
| Size | 21 | 21 | 163 |
| Primer 2 details |
|---|
| Sequence | TCAGTGGCAGACAACTTCTCT | GTCTGCCACTGAATTCGTTGA | Start: 59 |
| Tm | 58.958 | 58.857 | End: 220 |
| Size | 21 | 21 | 162 |
| Primer 3 details |
|---|
| Sequence | TCAGTGGCAGACAACCATTT | TGTCTGCCACTGAATTCGTTG | Start: 109 |
| Tm | 57.637 | 59.127 | End: 221 |
| Size | 20 | 21 | 113 |
| Primer 4 details |
|---|
| Sequence | CGAATTCAGTGGCAGACAACC | GCCACTGAATTCGTTGAAGAGA | Start: 104 |
| Tm | 59.802 | 58.933 | End: 216 |
| Size | 21 | 22 | 113 |
| Primer 5 details |
|---|
| Sequence | TCAGTGGCAGACAACTTCTCT | TGTCTGCCACTGAATTCGTT | Start: 59 |
| Tm | 58.958 | 57.745 | End: 221 |
| Size | 21 | 20 | 163 |
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