Primer Details for NODE_147728_length_447_cov_115.542781 |
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| (T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGAGTGTTCTGCATGGAGGA | ACTTTGGGTTTTAAAAGCTCACAGT | Start: 16 |
| Tm | 59.998 | 59.813 | End: 137 |
| Size | 21 | 25 | 122 |
| Primer 2 details |
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| Sequence | GGAGGAGATTCACCAATGGCA | ACTTTGGGTTTTAAAAGCTCACAGT | Start: 31 |
| Tm | 60.064 | 59.813 | End: 137 |
| Size | 21 | 25 | 107 |
| Primer 3 details |
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| Sequence | TGGAGGAGATTCACCAATGGC | ACTTTGGGTTTTAAAAGCTCACAGT | Start: 30 |
| Tm | 60.064 | 59.813 | End: 137 |
| Size | 21 | 25 | 108 |
| Primer 4 details |
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| Sequence | TGTGTGAGTGTTCTGCATGGA | ACTTTGGGTTTTAAAAGCTCACAGT | Start: 13 |
| Tm | 59.859 | 59.813 | End: 137 |
| Size | 21 | 25 | 125 |
| Primer 5 details |
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| Sequence | CTGCATGGAGGAGATTCACCA | ACTTTGGGTTTTAAAAGCTCACAGT | Start: 25 |
| Tm | 59.789 | 59.813 | End: 137 |
| Size | 21 | 25 | 113 |
