Primer Details for NODE_1475_length_259_cov_1.344086 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTTCACTGGCTCACTTAAGC | ACTAGGACAAACAAATAAAACAAAACA | Start: 88 |
| Tm | 58.92 | 57.069 | End: 196 |
| Size | 21 | 27 | 109 |
| Primer 2 details |
|---|
| Sequence | CCTTCACTGGCTCACTTAAGCT | ACTAGGACAAACAAATAAAACAAAACA | Start: 88 |
| Tm | 60.29 | 57.069 | End: 196 |
| Size | 22 | 27 | 109 |
| Primer 3 details |
|---|
| Sequence | CTTCACTGGCTCACTTAAGCT | ACTAGGACAAACAAATAAAACAAAACA | Start: 89 |
| Tm | 57.949 | 57.069 | End: 196 |
| Size | 21 | 27 | 108 |
| Primer 4 details |
|---|
| Sequence | CTTCACTGGCTCACTTAAGCTT | ACTAGGACAAACAAATAAAACAAAACA | Start: 89 |
| Tm | 58.593 | 57.069 | End: 196 |
| Size | 22 | 27 | 108 |
| Primer 5 details |
|---|
| Sequence | TTCACTGGCTCACTTAAGCTT | ACTAGGACAAACAAATAAAACAAAACA | Start: 90 |
| Tm | 57.508 | 57.069 | End: 196 |
| Size | 21 | 27 | 107 |
