Primer Details for NODE_145960_length_210_cov_1279.941606 |
|---|
| (T)12ctcagtacttctcagtacatggaataggatggaatagg(T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATGTTGGACATGCACAAC | AGCCTGTAATACTATTTTGAAACCCT | Start: 90 |
| Tm | 57.647 | 58.589 | End: 204 |
| Size | 20 | 26 | 115 |
| Primer 2 details |
|---|
| Sequence | TCTCAGTACATGGAATAGGATGGA | CACAATTTTACGTTGTGCATGTCC | Start: 21 |
| Tm | 58.284 | 59.852 | End: 120 |
| Size | 24 | 24 | 100 |
| Primer 3 details |
|---|
| Sequence | GGATGTTGGACATGCACAAC | GCCTGTAATACTATTTTGAAACCCT | Start: 90 |
| Tm | 57.647 | 57.359 | End: 203 |
| Size | 20 | 25 | 114 |
| Primer 4 details |
|---|
| Sequence | GGATGTTGGACATGCACAAC | AGCCTGTAATACTATTTTGAAACCC | Start: 90 |
| Tm | 57.647 | 57.359 | End: 204 |
| Size | 20 | 25 | 115 |
| Primer 5 details |
|---|
| Sequence | ACTTCTCAGTACATGGAATAGGATGG | CACAATTTTACGTTGTGCATGTCC | Start: 18 |
| Tm | 59.957 | 59.852 | End: 120 |
| Size | 26 | 24 | 103 |
