ssr_primer
Primer Details for NODE_145174_length_1408_cov_16.779775 |
|---|
| (CGC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGAGAAGCCGTATCGTCC | TCTCCATGTTGTTCTCCGCC | Start: 250 |
| Tm | 60.041 | 60.036 | End: 481 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GATACGGCTTCTCTCGCCTC | Start: 130 |
| Tm | 60.038 | 60.04 | End: 265 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GGACGATACGGCTTCTCTCG | Start: 130 |
| Tm | 60.038 | 60.041 | End: 269 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GAAGGAGAGGCGAGAGAAGC | TCTCCATGTTGTTCTCCGCC | Start: 240 |
| Tm | 59.898 | 60.036 | End: 481 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GCTTCTCTCGCCTCTCCTTC | Start: 130 |
| Tm | 60.038 | 59.898 | End: 259 |
| Size | 20 | 20 | 130 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGAGAAGCCGTATCGTCC | TCTCCATGTTGTTCTCCGCC | Start: 250 |
| Tm | 60.041 | 60.036 | End: 481 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GATACGGCTTCTCTCGCCTC | Start: 130 |
| Tm | 60.038 | 60.04 | End: 265 |
| Size | 20 | 20 | 136 |
| Primer 3 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GGACGATACGGCTTCTCTCG | Start: 130 |
| Tm | 60.038 | 60.041 | End: 269 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GAAGGAGAGGCGAGAGAAGC | TCTCCATGTTGTTCTCCGCC | Start: 240 |
| Tm | 59.898 | 60.036 | End: 481 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | CTCGCCGAAAGACAGGACTT | GCTTCTCTCGCCTCTCCTTC | Start: 130 |
| Tm | 60.038 | 59.898 | End: 259 |
| Size | 20 | 20 | 130 |
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