ssr_primer
Primer Details for NODE_145079_length_223_cov_2.446667 |
|---|
| (ACG)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACGGCTACATTTCCACGA | GGTCGTCGTCGTGGAAATGT | Start: 3 |
| Tm | 60.457 | 60.665 | End: 140 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CATTTCCACGACGACGACGA | TCGTGGAAATGTAGCCGTCG | Start: 12 |
| Tm | 60.727 | 60.457 | End: 172 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | CGACGGCTACATTTCCACGA | TCGTCGTCGTCGTGGAAATG | Start: 3 |
| Tm | 60.457 | 60.727 | End: 181 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | ATTTCCACGACGACGACGAC | TCGTGGAAATGTAGCCGTCG | Start: 13 |
| Tm | 60.727 | 60.457 | End: 172 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | ATTTCCACGACGACGACGAC | TCGTCGTGGAAATGTAGCCG | Start: 13 |
| Tm | 60.727 | 60.457 | End: 175 |
| Size | 20 | 20 | 163 |
(ACG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACGGCTACATTTCCACGA | GGTCGTCGTCGTGGAAATGT | Start: 3 |
| Tm | 60.457 | 60.665 | End: 140 |
| Size | 20 | 20 | 138 |
| Primer 2 details |
|---|
| Sequence | CATTTCCACGACGACGACGA | TCGTGGAAATGTAGCCGTCG | Start: 12 |
| Tm | 60.727 | 60.457 | End: 172 |
| Size | 20 | 20 | 161 |
| Primer 3 details |
|---|
| Sequence | CGACGGCTACATTTCCACGA | TCGTCGTCGTCGTGGAAATG | Start: 3 |
| Tm | 60.457 | 60.727 | End: 181 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
|---|
| Sequence | ATTTCCACGACGACGACGAC | TCGTGGAAATGTAGCCGTCG | Start: 13 |
| Tm | 60.727 | 60.457 | End: 172 |
| Size | 20 | 20 | 160 |
| Primer 5 details |
|---|
| Sequence | ATTTCCACGACGACGACGAC | TCGTCGTGGAAATGTAGCCG | Start: 13 |
| Tm | 60.727 | 60.457 | End: 175 |
| Size | 20 | 20 | 163 |
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