ssr_primer
Primer Details for NODE_1441_length_1126_cov_19.739791 |
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| (GT)5(G)(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGACGTGTGGAGTTTGCAGT | GCTTGAAATGTGGACAGCGG | Start: 322 |
| Tm | 60.108 | 60.11 | End: 447 |
| Size | 20 | 20 | 126 |
| Primer 2 details |
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| Sequence | CCGCTGTCCACATTTCAAGC | TTGAGTCGCGAATGAGTCCC | Start: 428 |
| Tm | 60.11 | 60.109 | End: 672 |
| Size | 20 | 20 | 245 |
| Primer 3 details |
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| Sequence | CTTGGCGTGTGTGTGTGTTT | GATCTCTGTCTGTGTGGCCC | Start: 899 |
| Tm | 59.83 | 60.108 | End: 1060 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
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| Sequence | CTTGGCGTGTGTGTGTGTTT | GGATCTCTGTCTGTGTGGCC | Start: 899 |
| Tm | 59.83 | 60.108 | End: 1061 |
| Size | 20 | 20 | 163 |
| Primer 5 details |
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| Sequence | GGGACTCATTCGCGACTCAA | AAACACACACACACGCCAAG | Start: 653 |
| Tm | 60.109 | 59.83 | End: 918 |
| Size | 20 | 20 | 266 |
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