ssr_primer
Primer Details for NODE_140190_length_1702_cov_25.110497 |
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| (TCA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTCTGGCGAGATCCCTTCT | GCAGGAAATCGCAGGCAATT | Start: 1457 |
| Tm | 60.033 | 59.826 | End: 1604 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
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| Sequence | CCTCGTGCACTTCGACAAAC | CAGTACCTGCCAGACAGCAA | Start: 539 |
| Tm | 59.768 | 59.965 | End: 656 |
| Size | 20 | 20 | 118 |
| Primer 3 details |
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| Sequence | TTCCAGAGAACCCAGTTCGC | CTCCCCTTTTGTTGCGAGGA | Start: 252 |
| Tm | 59.965 | 60.251 | End: 369 |
| Size | 20 | 20 | 118 |
| Primer 4 details |
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| Sequence | AGTCTGGCGAGATCCCTTCT | ATAAGTCTGCCACCCCCTCA | Start: 1457 |
| Tm | 60.033 | 60.253 | End: 1624 |
| Size | 20 | 20 | 168 |
| Primer 5 details |
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| Sequence | AGTCTGGCGAGATCCCTTCT | TGATAAGTCTGCCACCCCCT | Start: 1457 |
| Tm | 60.033 | 60.253 | End: 1626 |
| Size | 20 | 20 | 170 |
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