Primer Details for NODE_139882_length_204_cov_372.129771 |
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| (ATT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCCTCCCCCAACACACTAT | TGTCTGTTGAATTATTTTTCCTCAGCT | Start: 33 |
| Tm | 60.179 | 59.769 | End: 147 |
| Size | 20 | 27 | 115 |
| Primer 2 details |
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| Sequence | AGTTGACCCTGCAAAACCCT | TGTCTGTTGAATTATTTTTCCTCAGCT | Start: 18 |
| Tm | 59.738 | 59.769 | End: 147 |
| Size | 20 | 27 | 130 |
| Primer 3 details |
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| Sequence | ACCCTCCCCCAACACACTAT | TGTCTGTTGAATTATTTTTCCTCAGC | Start: 33 |
| Tm | 60.179 | 58.63 | End: 147 |
| Size | 20 | 26 | 115 |
| Primer 4 details |
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| Sequence | CAAAACCCTCCCCCAACACA | TGTCTGTTGAATTATTTTTCCTCAGCT | Start: 29 |
| Tm | 60.398 | 59.769 | End: 147 |
| Size | 20 | 27 | 119 |
| Primer 5 details |
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| Sequence | AAAACCCTCCCCCAACACAC | TGTCTGTTGAATTATTTTTCCTCAGCT | Start: 30 |
| Tm | 60.398 | 59.769 | End: 147 |
| Size | 20 | 27 | 118 |
