ssr_primer
Primer Details for NODE_139136_length_1439_cov_15.270864 |
|---|
| (T)10aaacaaatacgcaaaaataaatatttttcctac(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAGCGTCGTGAGTAGG | GACCCTGCTGATGGAACCAA | Start: 68 |
| Tm | 60.039 | 59.962 | End: 241 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
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| Sequence | CTCTACAGCAGCGTGTCGAT | CATCATGCCCACCACCTTCT | Start: 1181 |
| Tm | 59.9 | 60.034 | End: 1369 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
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| Sequence | GGAAGAGGGACGAGGCTCTA | CATCATGCCCACCACCTTCT | Start: 1166 |
| Tm | 60.107 | 60.034 | End: 1369 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
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| Sequence | AGAGGGACGAGGCTCTACAG | CATCATGCCCACCACCTTCT | Start: 1169 |
| Tm | 60.107 | 60.034 | End: 1369 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | AGGTCAAAGCAGTCACCCTG | GACCCTGCTGATGGAACCAA | Start: 85 |
| Tm | 59.891 | 59.962 | End: 241 |
| Size | 20 | 20 | 157 |
(T)14ccccaataca(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAGCGTCGTGAGTAGG | GACCCTGCTGATGGAACCAA | Start: 68 |
| Tm | 60.039 | 59.962 | End: 241 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | CTCTACAGCAGCGTGTCGAT | CATCATGCCCACCACCTTCT | Start: 1181 |
| Tm | 59.9 | 60.034 | End: 1369 |
| Size | 20 | 20 | 189 |
| Primer 3 details |
|---|
| Sequence | GGAAGAGGGACGAGGCTCTA | CATCATGCCCACCACCTTCT | Start: 1166 |
| Tm | 60.107 | 60.034 | End: 1369 |
| Size | 20 | 20 | 204 |
| Primer 4 details |
|---|
| Sequence | AGAGGGACGAGGCTCTACAG | CATCATGCCCACCACCTTCT | Start: 1169 |
| Tm | 60.107 | 60.034 | End: 1369 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | AGGTCAAAGCAGTCACCCTG | GACCCTGCTGATGGAACCAA | Start: 85 |
| Tm | 59.891 | 59.962 | End: 241 |
| Size | 20 | 20 | 157 |
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