Primer Details for NODE_138_length_215_cov_16.788732 | |||
|---|---|---|---|
| (A)14: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCACAGGAGAACCTTCTTAAACCT | TCAAGGAGAACTGAACAGGCA | Start: 38 |
| Tm | 59.341 | 59.232 | End: 171 |
| Size | 24 | 21 | 134 |
| Primer 2 details | |||
| Sequence | TCACAGGAGAACCTTCTTAAACCT | TCTCAAGGAGAACTGAACAGGC | Start: 38 |
| Tm | 59.341 | 59.962 | End: 173 |
| Size | 24 | 22 | 136 |
| Primer 3 details | |||
| Sequence | TCACAGGAGAACCTTCTTAAACC | TCAAGGAGAACTGAACAGGCA | Start: 38 |
| Tm | 58.031 | 59.232 | End: 171 |
| Size | 23 | 21 | 134 |
| Primer 4 details | |||
| Sequence | CACAGGAGAACCTTCTTAAACCT | TCAAGGAGAACTGAACAGGCA | Start: 39 |
| Tm | 58.026 | 59.232 | End: 171 |
| Size | 23 | 21 | 133 |
| Primer 5 details | |||
| Sequence | TGTCCACATATATCACAGGAGAACC | TCAAGGAGAACTGAACAGGCA | Start: 26 |
| Tm | 59.93 | 59.232 | End: 171 |
| Size | 25 | 21 | 146 |
