Primer Details for NODE_135466_length_211_cov_157.949275 |
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| (T)10ggtctgattaggtctgattacagtttatttcagttta(T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTCTGATTAGGTCTGATTACAGT | GCTTTTTGCGTATGTGTTTTATGT | Start: 9 |
| Tm | 59.042 | 57.553 | End: 189 |
| Size | 25 | 24 | 181 |
| Primer 2 details |
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| Sequence | TGGTCTGATTAGGTCTGATTACAGT | TCCAAAACGTTCTTTGATTTGTTCT | Start: 9 |
| Tm | 59.042 | 58.374 | End: 133 |
| Size | 25 | 25 | 125 |
| Primer 3 details |
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| Sequence | TGGTCTGATTAGGTCTGATTACAGT | TCCAAAACGTTCTTTGATTTGTTC | Start: 9 |
| Tm | 59.042 | 57.121 | End: 133 |
| Size | 25 | 24 | 125 |
| Primer 4 details |
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| Sequence | TGGTCTGATTAGGTCTGATTACAGT | CCAAAACGTTCTTTGATTTGTTCT | Start: 9 |
| Tm | 59.042 | 57.114 | End: 132 |
| Size | 25 | 24 | 124 |
| Primer 5 details |
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| Sequence | TGGTCTGATTAGGTCTGATTACAGTT | GCTTTTTGCGTATGTGTTTTATGT | Start: 9 |
| Tm | 59.559 | 57.553 | End: 189 |
| Size | 26 | 24 | 181 |
