Primer Details for NODE_134492_length_250_cov_17.728814 | |||
|---|---|---|---|
| (A)22: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGACATTCAACTTTTGTTCTTTCTGC | TGAGTGAACAGGTAAGAACAGGT | Start: 65 |
| Tm | 60.02 | 59.29 | End: 224 |
| Size | 26 | 23 | 160 |
| Primer 2 details | |||
| Sequence | TCAACTTTTGTTCTTTCTGCAAGT | TGAGTGAACAGGTAAGAACAGGT | Start: 71 |
| Tm | 57.975 | 59.29 | End: 224 |
| Size | 24 | 23 | 154 |
| Primer 3 details | |||
| Sequence | TGGAAAACAATTGGACATTCAACT | TGAGTGAACAGGTAAGAACAGGT | Start: 53 |
| Tm | 57.767 | 59.29 | End: 224 |
| Size | 24 | 23 | 172 |
| Primer 4 details | |||
| Sequence | GGACATTCAACTTTTGTTCTTTCTGC | GTGAGTGAACAGGTAAGAACAGG | Start: 65 |
| Tm | 60.02 | 58.941 | End: 225 |
| Size | 26 | 23 | 161 |
| Primer 5 details | |||
| Sequence | TCAACTTTTGTTCTTTCTGCAAGT | GTGAGTGAACAGGTAAGAACAGG | Start: 71 |
| Tm | 57.975 | 58.941 | End: 225 |
| Size | 24 | 23 | 155 |
