Primer Details for NODE_132631_length_248_cov_66.085714 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CACTGGTGTGTTAATACGTGTATAACA | ACGTGACAGCAGAGATGATACC | Start: 113 |
| Tm | 59.671 | 59.9 | End: 231 |
| Size | 27 | 22 | 119 |
| Primer 2 details |
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| Sequence | ACGTGTATAACATGTAACAGACTTCA | ACGTGACAGCAGAGATGATACC | Start: 128 |
| Tm | 58.627 | 59.9 | End: 231 |
| Size | 26 | 22 | 104 |
| Primer 3 details |
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| Sequence | ACTGGTGTGTTAATACGTGTATAACA | ACGTGACAGCAGAGATGATACC | Start: 114 |
| Tm | 58.623 | 59.9 | End: 231 |
| Size | 26 | 22 | 118 |
| Primer 4 details |
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| Sequence | CACTGGTGTGTTAATACGTGTATAACA | CGTGACAGCAGAGATGATACCT | Start: 113 |
| Tm | 59.671 | 59.64 | End: 230 |
| Size | 27 | 22 | 118 |
| Primer 5 details |
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| Sequence | ACGTGTATAACATGTAACAGACTTCA | CGTGACAGCAGAGATGATACCT | Start: 128 |
| Tm | 58.627 | 59.64 | End: 230 |
| Size | 26 | 22 | 103 |
