ssr_primer
Primer Details for NODE_124193_length_467_cov_42.913706 |
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| (ATTT)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGACAGAGTTCTGACACCCA | GGAGTGGCGAAGAGAGCAAT | Start: 4 |
| Tm | 59.326 | 60.108 | End: 280 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
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| Sequence | TCGACAGAGTTCTGACACCC | GGAGTGGCGAAGAGAGCAAT | Start: 3 |
| Tm | 59.04 | 60.108 | End: 280 |
| Size | 20 | 20 | 278 |
| Primer 3 details |
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| Sequence | TCCCGCTTTCTGTGATGAGT | GGAGTGGCGAAGAGAGCAAT | Start: 142 |
| Tm | 59.023 | 60.108 | End: 280 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
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| Sequence | CGACAGAGTTCTGACACCCA | GAAGGAGTGGCGAAGAGAGC | Start: 4 |
| Tm | 59.326 | 60.459 | End: 283 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
|---|
| Sequence | TCCCGCTTTCTGTGATGAGT | GAAGGAGTGGCGAAGAGAGC | Start: 142 |
| Tm | 59.023 | 60.459 | End: 283 |
| Size | 20 | 20 | 142 |
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