Primer Details for NODE_123767_length_338_cov_217.116981 | |||
|---|---|---|---|
| (A)13: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCATTACATCTACCTTGCTGA | AGAAAAATGCCATTGACCAGCT | Start: 59 |
| Tm | 57.514 | 59.098 | End: 160 |
| Size | 22 | 22 | 102 |
| Primer 2 details | |||
| Sequence | CCAAGTCAGTAACCAGAGGGC | CACCTTGCTAATACAAAGTCTTTCCA | Start: 90 |
| Tm | 60.339 | 59.793 | End: 197 |
| Size | 21 | 26 | 108 |
| Primer 3 details | |||
| Sequence | CCAAGTCAGTAACCAGAGGGC | ACCTTGCTAATACAAAGTCTTTCCA | Start: 90 |
| Tm | 60.339 | 58.696 | End: 196 |
| Size | 21 | 25 | 107 |
| Primer 4 details | |||
| Sequence | TGCATTACATCTACCTTGCTGAA | AGAAAAATGCCATTGACCAGCT | Start: 59 |
| Tm | 58.157 | 59.098 | End: 160 |
| Size | 23 | 22 | 102 |
| Primer 5 details | |||
| Sequence | TTGCATTACATCTACCTTGCTGA | AGAAAAATGCCATTGACCAGCT | Start: 58 |
| Tm | 58.157 | 59.098 | End: 160 |
| Size | 23 | 22 | 103 |
