ssr_primer
Primer Details for NODE_1174_length_1782_cov_366.283207 |
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| (A)12taaagaaaaataaagaaatatattttgctatattttgc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGGTATTTGAACCCAGGC | TAACGACTAACGAGACGCCG | Start: 24 |
| Tm | 60.035 | 59.905 | End: 209 |
| Size | 20 | 20 | 186 |
| Primer 2 details |
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| Sequence | CGGCGTCTCGTTAGTCGTTA | GGAACGCTTCCTTCCAGTGA | Start: 190 |
| Tm | 59.905 | 59.965 | End: 335 |
| Size | 20 | 20 | 146 |
| Primer 3 details |
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| Sequence | GGTATTTGAACCCAGGCCCA | TAACGACTAACGAGACGCCG | Start: 27 |
| Tm | 59.96 | 59.905 | End: 209 |
| Size | 20 | 20 | 183 |
| Primer 4 details |
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| Sequence | TATGTCGGCTGTGCGTCAAT | ATAGCTGCCACCCTACCTCA | Start: 1029 |
| Tm | 60.108 | 60.031 | End: 1244 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
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| Sequence | TATGTCGGCTGTGCGTCAAT | TGATAGCTGCCACCCTACCT | Start: 1029 |
| Tm | 60.108 | 60.031 | End: 1246 |
| Size | 20 | 20 | 218 |
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