Primer Details for NODE_117339_length_252_cov_12.787709 | |||
|---|---|---|---|
| (T)15: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATCAGTGATCATCAGTGGTT | AGTGGTGGTAGATTTTCTTCTTGT | Start: 85 |
| Tm | 57.168 | 58.058 | End: 213 |
| Size | 22 | 24 | 129 |
| Primer 2 details | |||
| Sequence | TCATCAGTGATCATCAGTGGTTT | AGTGGTGGTAGATTTTCTTCTTGT | Start: 85 |
| Tm | 57.83 | 58.058 | End: 213 |
| Size | 23 | 24 | 129 |
| Primer 3 details | |||
| Sequence | TCATCAGTGATCATCAGTGGTT | AGTGGTGGTAGATTTTCTTCTTGTT | Start: 85 |
| Tm | 57.168 | 58.634 | End: 213 |
| Size | 22 | 25 | 129 |
| Primer 4 details | |||
| Sequence | TCATCAGTGATCATCAGTGGTT | AAGTGGTGGTAGATTTTCTTCTTGT | Start: 85 |
| Tm | 57.168 | 58.634 | End: 214 |
| Size | 22 | 25 | 130 |
| Primer 5 details | |||
| Sequence | ATCATCAGTGATCATCAGTGGTT | AGTGGTGGTAGATTTTCTTCTTGT | Start: 84 |
| Tm | 57.631 | 58.058 | End: 213 |
| Size | 23 | 24 | 130 |
