ssr_primer
Primer Details for NODE_114496_length_2671_cov_6.384911 |
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| (T)10gggtgcaaatgggtgcaa(ATTTT)5(T)11*: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGAATGGCACTCAACTCCG | CATGCTGCTCCAGGAGTGAA | Start: 2197 |
| Tm | 60.322 | 60.036 | End: 2466 |
| Size | 20 | 20 | 270 |
| Primer 2 details |
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| Sequence | TAAAGCTTCCGGCAGATGGG | CACTTGTGATGCTTGGCGTT | Start: 682 |
| Tm | 60.107 | 59.688 | End: 874 |
| Size | 20 | 20 | 193 |
| Primer 3 details |
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| Sequence | TTTGGCCCTGAAAGTTGGCT | AGCCCAGAGCCTCAACTCTA | Start: 1701 |
| Tm | 60.398 | 59.958 | End: 1950 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
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| Sequence | ACGCCACAAATCTCCCTGAA | AGCCCAGAGCCTCAACTCTA | Start: 1729 |
| Tm | 59.6 | 59.958 | End: 1950 |
| Size | 20 | 20 | 222 |
| Primer 5 details |
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| Sequence | TGGAATGGCACTCAACTCCG | ACGTAGTCATGCTGCTCCAG | Start: 2197 |
| Tm | 60.322 | 59.826 | End: 2473 |
| Size | 20 | 20 | 277 |
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