Primer Details for NODE_114106_length_229_cov_37.282051 | |||
|---|---|---|---|
| (A)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGGATGAAGTGAAAACAGAAGTT | TGAGTCAGAACTGTGTCAGCT | Start: 0 |
| Tm | 57.374 | 58.965 | End: 140 |
| Size | 24 | 21 | 141 |
| Primer 2 details | |||
| Sequence | AGGATGAAGTGAAAACAGAAGTTACA | TGAGTCAGAACTGTGTCAGCT | Start: 1 |
| Tm | 58.946 | 58.965 | End: 140 |
| Size | 26 | 21 | 140 |
| Primer 3 details | |||
| Sequence | GAGGATGAAGTGAAAACAGAAGTT | TGAGTCAGAACTGTGTCAGC | Start: 0 |
| Tm | 57.374 | 57.484 | End: 140 |
| Size | 24 | 20 | 141 |
| Primer 4 details | |||
| Sequence | GAGGATGAAGTGAAAACAGAAGTT | ATGAGTCAGAACTGTGTCAGCT | Start: 0 |
| Tm | 57.374 | 59.368 | End: 141 |
| Size | 24 | 22 | 142 |
| Primer 5 details | |||
| Sequence | GAGGATGAAGTGAAAACAGAAGTTACA | TGAGTCAGAACTGTGTCAGCT | Start: 0 |
| Tm | 59.773 | 58.965 | End: 140 |
| Size | 27 | 21 | 141 |
