Primer Details for NODE_110129_length_263_cov_17.257895 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGTATTTGGTGCACTTTGT | ACTGCTAAATCTGTATTTGTGAAGGT | Start: 15 |
| Tm | 58.422 | 59.057 | End: 185 |
| Size | 21 | 26 | 171 |
| Primer 2 details |
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| Sequence | TCCGTATTTGGTGCACTTTGT | TTTTCCAAAGACTGCTAAATCTGT | Start: 15 |
| Tm | 58.422 | 57.04 | End: 195 |
| Size | 21 | 24 | 181 |
| Primer 3 details |
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| Sequence | TCCGTATTTGGTGCACTTTGT | TGTACTTTTGAACACACTCCATATGT | Start: 15 |
| Tm | 58.422 | 59.004 | End: 122 |
| Size | 21 | 26 | 108 |
| Primer 4 details |
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| Sequence | TCCGTATTTGGTGCACTTTGT | GACTGCTAAATCTGTATTTGTGAAGGT | Start: 15 |
| Tm | 58.422 | 59.88 | End: 186 |
| Size | 21 | 27 | 172 |
| Primer 5 details |
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| Sequence | TCCGTATTTGGTGCACTTTGT | TGATTTTCCAAAGACTGCTAAATCTGT | Start: 15 |
| Tm | 58.422 | 59.769 | End: 198 |
| Size | 21 | 27 | 184 |
