Primer Details for NODE_109401_length_215_cov_102.091549 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTGGTTGTAATGATGCCCC | AGAGCTCATTGAAACTTATTGAAACT | Start: 69 |
| Tm | 60.342 | 57.599 | End: 193 |
| Size | 21 | 26 | 125 |
| Primer 2 details |
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| Sequence | CTGGTTGTAATGATGCCCCC | AGAGCTCATTGAAACTTATTGAAACT | Start: 71 |
| Tm | 58.597 | 57.599 | End: 193 |
| Size | 20 | 26 | 123 |
| Primer 3 details |
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| Sequence | CCTGGTTGTAATGATGCCCC | AGAGCTCATTGAAACTTATTGAAACT | Start: 70 |
| Tm | 58.597 | 57.599 | End: 193 |
| Size | 20 | 26 | 124 |
| Primer 4 details |
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| Sequence | ACCTGGTTGTAATGATGCCCC | AGAGCTCATTGAAACTTATTGAAACTT | Start: 69 |
| Tm | 60.342 | 58.146 | End: 193 |
| Size | 21 | 27 | 125 |
| Primer 5 details |
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| Sequence | ACCTGGTTGTAATGATGCCCC | AAGAGCTCATTGAAACTTATTGAAACT | Start: 69 |
| Tm | 60.342 | 58.146 | End: 194 |
| Size | 21 | 27 | 126 |
