Primer Details for NODE_107000_length_474_cov_2.600998 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCCAGTCTTGATGAGGAGC | TCAGTTCATGTATTGGAGTTGCT | Start: 83 |
| Tm | 59.749 | 58.09 | End: 243 |
| Size | 20 | 23 | 161 |
| Primer 2 details | |||
| Sequence | AGCAACTCCAATACATGAACTGA | AGGCACCTTGTTTCACCCTT | Start: 221 |
| Tm | 58.09 | 59.738 | End: 458 |
| Size | 23 | 20 | 238 |
| Primer 3 details | |||
| Sequence | AGCAACTCCAATACATGAACTGA | AAGGCACCTTGTTTCACCCT | Start: 221 |
| Tm | 58.09 | 59.738 | End: 459 |
| Size | 23 | 20 | 239 |
| Primer 4 details | |||
| Sequence | TGCCAGTCTTGATGAGGAGC | TGCTCTCTTCAGTTCATGTATTGGA | Start: 83 |
| Tm | 59.749 | 60.046 | End: 251 |
| Size | 20 | 25 | 169 |
| Primer 5 details | |||
| Sequence | TCCAATACATGAACTGAAGAGAGCA | AGGCACCTTGTTTCACCCTT | Start: 227 |
| Tm | 60.046 | 59.738 | End: 458 |
| Size | 25 | 20 | 232 |
