ssr_primer
Primer Details for NODE_103203_length_986_cov_4.481928 |
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| (TCC)5tttggttgaacgccctgagttaaaaattattttgcaaaag(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TAGCAAAAGAGGGAGGGGGA | TTTGTCAGTGGTAGTGGCCC | Start: 677 |
| Tm | 59.88 | 59.89 | End: 778 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
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| Sequence | AATTGGGGCCACTACCACTG | GATGTCTGCCGCTCCACTAG | Start: 754 |
| Tm | 59.96 | 60.249 | End: 966 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
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| Sequence | AATTGGGGCCACTACCACTG | ACGCCACACTATGCAGATGT | Start: 754 |
| Tm | 59.96 | 59.75 | End: 981 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
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| Sequence | CACACTTCATCTGTGGCCCA | CAGTGGTAGTGGCCCCAATT | Start: 563 |
| Tm | 60.251 | 59.96 | End: 773 |
| Size | 20 | 20 | 211 |
| Primer 5 details |
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| Sequence | ACACACTTCATCTGTGGCCC | CAGTGGTAGTGGCCCCAATT | Start: 562 |
| Tm | 60.251 | 59.96 | End: 773 |
| Size | 20 | 20 | 212 |
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