Primer Details for NODE_101433_length_278_cov_33.282927 |
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| (TTA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAATGGAATGTACCAAAATTAGCCT | CCTGTGCTGCTATAAGAACGC | Start: 136 |
| Tm | 59.333 | 59.405 | End: 235 |
| Size | 26 | 21 | 100 |
| Primer 2 details |
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| Sequence | TCAATGGAATGTACCAAAATTAGCC | CCTGTGCTGCTATAAGAACGC | Start: 136 |
| Tm | 58.127 | 59.405 | End: 235 |
| Size | 25 | 21 | 100 |
| Primer 3 details |
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| Sequence | TCAATGGAATGTACCAAAATTAGCCTT | CCTGTGCTGCTATAAGAACGC | Start: 136 |
| Tm | 59.82 | 59.405 | End: 235 |
| Size | 27 | 21 | 100 |
| Primer 4 details |
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| Sequence | ATCAATGGAATGTACCAAAATTAGCCT | CCTGTGCTGCTATAAGAACGC | Start: 135 |
| Tm | 59.655 | 59.405 | End: 235 |
| Size | 27 | 21 | 101 |
| Primer 5 details |
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| Sequence | ATCAATGGAATGTACCAAAATTAGCC | CCTGTGCTGCTATAAGAACGC | Start: 135 |
| Tm | 58.5 | 59.405 | End: 235 |
| Size | 26 | 21 | 101 |
