About TiGeR

Tilletia indica Mitra is restricted fungal pathogen causing Karnal bunt (KB) of wheat results in 0.3-0.5% yield loss in India. A study carried out the genome assemblies and annotations of three T. indica strains using PacBio and Illumina reads extracted from cultures of PSWKBGH-1 and PSWKBGH-2 (both non-pathogenic monosporidial lines) along with PSWKBGH-3 (pathogenic dikaryon stage of 1 & 2 monosporidial lines) isolated from infected wheat grains. A sum of 367, 482 and 8812 scaffolds with genome sizes of 37, 37 and 43 Mb were generated for PSWKBGH-1, 2 and 3 respectively, submitted to NCBI. A sum of 10203, 10304 and 13413 genes were predicted in genome assemblies of PSWKBGH-1, 2 and 3 respectively. Genomic and polymorphic SSRs were also extracted from all three genome assmblies. This study also analysed transcriptome data isolated from infected wheat grains at 24 hours, 48 hours and 7 days after inoculation of PSWKBGH-1, 2 and 3 for the identification of T. indica genes in dikaryon stage which are responsible for causing the pathogenicity in wheat and for the identification of wheat genes which are affected during pathogenic dikaryon stage of T. indica (PSWKBGH-3). A sum of 21, 35 and 134 genes at 24hai, 48hai and 7dai respectively of T.indica activated only in dikaryon stage, and 54, 529 and 87 genes at 24hai, 48hai and 7dai respectively of T.indica upregulated in dikaryon stage were found putative pathogenicity related genes. A sum of 23, 17 and 52 wheat genes at 24hai, 48hai and 7dai respectively were upregulated due to presence of dikaryon stage only. The results obtained during this study were compiled in to TiGeR, which could be a first resource for T.indica to be utilized by research community to prepare KB resistance wheat varieties and in understanding plant pathogen interaction during progression of KB in wheat.