Primer Details for TRINITY_DN713_c0_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCTGTGGAACTCACCAAAA | TGTTTTGTTTCCAACTTTCCAAACA | Start: 48 |
| Tm | 57.565 | 58.944 | End: 224 |
| Size | 20 | 25 | 177 |
| Primer 2 details |
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| Sequence | GCTGTGGAACTCACCAAAATCA | TGTTTTGTTTCCAACTTTCCAAACA | Start: 49 |
| Tm | 59.377 | 58.944 | End: 224 |
| Size | 22 | 25 | 176 |
| Primer 3 details |
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| Sequence | AGCTGTGGAACTCACCAAAATC | TGTTTTGTTTCCAACTTTCCAAACA | Start: 48 |
| Tm | 59.113 | 58.944 | End: 224 |
| Size | 22 | 25 | 177 |
| Primer 4 details |
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| Sequence | AGCTGTGGAACTCACCAAAA | TTGTTTTGTTTCCAACTTTCCAAACA | Start: 48 |
| Tm | 57.565 | 59.458 | End: 225 |
| Size | 20 | 26 | 178 |
| Primer 5 details |
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| Sequence | AGCTGTGGAACTCACCAAAA | GTTTTGTTTCCAACTTTCCAAACA | Start: 48 |
| Tm | 57.565 | 57.453 | End: 223 |
| Size | 20 | 24 | 176 |
