ssr_primer
Primer Details for TRINITY_DN6180_c0_g1_i2 |
|---|
| (AT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCAAAAGCCATCGCCTCT | CCACGGTGCCAACCTCTTAT | Start: 575 |
| Tm | 59.962 | 60.035 | End: 692 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | CCTTTCCAAAAGCCATCGCC | CCACGGTGCCAACCTCTTAT | Start: 572 |
| Tm | 60.109 | 60.035 | End: 692 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CGCCTTCTCTCGTCGGATAC | GCCTCCATATCTCTCCCCCA | Start: 633 |
| Tm | 60.041 | 60.179 | End: 889 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | GAAGTCTCCGCCTTCTCTCG | GCCTCCATATCTCTCCCCCA | Start: 625 |
| Tm | 59.9 | 60.179 | End: 889 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TTCCAAAAGCCATCGCCTCT | TTATCCTCCACCGTCGTCCA | Start: 575 |
| Tm | 59.962 | 60.324 | End: 709 |
| Size | 20 | 20 | 135 |
(TA)7: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCCAAAAGCCATCGCCTCT | CCACGGTGCCAACCTCTTAT | Start: 575 |
| Tm | 59.962 | 60.035 | End: 692 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | CCTTTCCAAAAGCCATCGCC | CCACGGTGCCAACCTCTTAT | Start: 572 |
| Tm | 60.109 | 60.035 | End: 692 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
|---|
| Sequence | CGCCTTCTCTCGTCGGATAC | GCCTCCATATCTCTCCCCCA | Start: 633 |
| Tm | 60.041 | 60.179 | End: 889 |
| Size | 20 | 20 | 257 |
| Primer 4 details |
|---|
| Sequence | GAAGTCTCCGCCTTCTCTCG | GCCTCCATATCTCTCCCCCA | Start: 625 |
| Tm | 59.9 | 60.179 | End: 889 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TTCCAAAAGCCATCGCCTCT | TTATCCTCCACCGTCGTCCA | Start: 575 |
| Tm | 59.962 | 60.324 | End: 709 |
| Size | 20 | 20 | 135 |
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