Primer Details for TRINITY_DN39889_c0_g1_i1 |
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| (AT)7: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTCGTATAATTCACTTCTCCCA | AACCCAATTCAATCAAATAGTTGTTGA | Start: 79 |
| Tm | 58.115 | 58.572 | End: 178 |
| Size | 25 | 27 | 100 |
| Primer 2 details |
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| Sequence | TGTTCGTATAATTCACTTCTCCCA | AAACCCAATTCAATCAAATAGTTGTTG | Start: 80 |
| Tm | 57.711 | 57.945 | End: 179 |
| Size | 24 | 27 | 100 |
| Primer 3 details |
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| Sequence | ATGTTCGTATAATTCACTTCTCCCA | AACCCAATTCAATCAAATAGTTGTTG | Start: 79 |
| Tm | 58.115 | 57.394 | End: 178 |
| Size | 25 | 26 | 100 |
| Primer 4 details |
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| Sequence | TGTTCGTATAATTCACTTCTCCCA | AAAACCCAATTCAATCAAATAGTTGTT | Start: 80 |
| Tm | 57.711 | 57.374 | End: 180 |
| Size | 24 | 27 | 101 |
| Primer 5 details |
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| Sequence | TGTTCGTATAATTCACTTCTCCCA | AAAAACCCAATTCAATCAAATAGTTGT | Start: 80 |
| Tm | 57.711 | 57.374 | End: 181 |
| Size | 24 | 27 | 102 |
